A Study on the Photobleaching Effect of 5-ALA Conjugated Gold Nanoparticles in a CT26 Tumor Model During Photodynamic Therapy
Authors
Abstract:
Introduction During the process photodynamic therapy (PDT), bleaching of photosensitizer induced by irradiation and generation of reactive oxygen species (ROS) can provide some information concerning the efficiency of treatment. Since gold nanoparticles (GNPs) have been highlighted as efficient drug delivery systems, in this study, by utilizing GNPs conjugated with 5 aminolevulinic acid (5-ALA-GNPs), the photobleaching of ALA-induced protoporphyrin IX (PpIX) was estimated on a colon carcinoma tumor model. Materials and Methods CT26 tumor models were prepared by subcutaneous injection of 5×105 cells into the right flank of Balb/c inbred mice. To estimate the time required to reach maximum concentration of PpIX in the tumors, the fluorescence signal of PpIX was monitored and PDT was performed by intratumoral injection of 5-ALA-GNPs, GNPs, and 5-ALA in separated groups for 15 min with a cycle of 5 min irradiation and 1 min darkness. The photobleaching rate was calculated from recorded fluorescence signals at the darkness intervals. Results The maximum fluorescence of PpIX was recorded 3 and 5 hr after injection of 5-ALA and 5-ALA-GNPs, respectively. Despite the low PpIX accumulation in tumors receiving conjugate, the photobleaching rate of PpIX was determined to be higher than 5-ALA. The reduction of the fluorescence signal due to 5-ALA-GNPs clearance was higher than that of 5-ALA. Conclusion Administration of 5-ALA-GNPs, intensification of ROS generating and the subsequent elevation of photobleaching results in higher treatment efficiency. Also, more rapid clearance of PpIX has an important implication in clinical application of 5-ALA-GNPs that decreases the undesirable effects on healthy tissues.
similar resources
a study on the photobleaching effect of 5-ala conjugated gold nanoparticles in a ct26 tumor model during photodynamic therapy
introduction during the process photodynamic therapy (pdt), bleaching of photosensitizer induced by irradiation and generation of reactive oxygen species (ros) can provide some information concerning the efficiency of treatment. since gold nanoparticles (gnps) have been highlighted as efficient drug delivery systems, in this study, by utilizing gnps conjugated with 5 aminolevulinic acid (5-ala-...
full textSonodynamic Therapy Using Protoporphyrin IX Conjugated to Gold Nanoparticles: An In Vivo Study on a Colon Tumor Model
Objective(s) Sonodynamic therapy is a physical treatment which utilizes ultrasound waves with an appropriate sensitizer such as protoporphyrin IX (PpIX). The activation of sensitizer depends on cavitation, and therefore, high intensity ultrasound is an important necessity. Beside, high intensity ultrasound can induce side effects on the healthy tissues which have surrounded tumor. The particle...
full textthe effect of consciousness raising (c-r) on the reduction of translational errors: a case study
در دوره های آموزش ترجمه استادان بیشتر سعی دارند دانشجویان را با انواع متون آشنا سازند، درحالی که کمتر به خطاهای مکرر آنان در متن ترجمه شده می پردازند. اهمیت تحقیق حاضر مبنی بر ارتکاب مکرر خطاهای ترجمانی حتی بعد از گذراندن دوره های تخصصی ترجمه از سوی دانشجویان است. هدف از آن تاکید بر خطاهای رایج میان دانشجویان مترجمی و کاهش این خطاها با افزایش آگاهی و هوشیاری دانشجویان از بروز آنها است.از آنجا ک...
15 صفحه اولa study on insurer solvency by panel data model: the case of iranian insurance market
the aim of this thesis is an approach for assessing insurer’s solvency for iranian insurance companies. we use of economic data with both time series and cross-sectional variation, thus by using the panel data model will survey the insurer solvency.
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
reflections on taught courses of the iranian ma program in english translation: a mixed-methods study
the issue of curriculum and syllabus evaluation and revision has been in center of attention right from when curriculum came into attention of educational institutions. thus everywhere in the world in educational institutions curricula and syllabi are evaluated and revised based on the goals, the needs, existing content, etc.. in iran any curriculum is designed in a committee of specialists and...
My Resources
Journal title
volume 9 issue 3
pages 217- 224
publication date 2012-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023